For a couple of days at room temperature or refrigerated for the grub season 2011 about 4 months. thanks

I've read that too, that the soil should be in the 10-15 degree range before applying the nematodes. I think it has more to do with making sure the grub larvae are nice and close to the surface so the nematodes can find them more easily; after all, the nematodes are stored in fridges that are a lot cooler than ten degrees.

Wait for lawn to thaw out i.e. mid to late Spring. Ground should be wet so predatory nematodes 'can swim' to the grubs/ asian lili beetle larva... Applied with clean hose attachment sprayer (that has no insecticide residue). May irrigate after application of Grub Busters to help nematodes travel down to grubs. thanks

I bought the nematodes & the sprayer attachment for my garden hose. The nematodes come in a sponge -- you fill the sprayer attachment with water and put the sponge in the water. Secure the lid and shake it up for a minute or two. Attach the garden hose and start spraying, beginning with the most affected areas of your lawn. You'll notice the water level in the sprayer attachment decreasing as you spray; you're finished when it is. And yes, all of this was in the instructions that came with the nematodes.

Oh, before you start all of that, try to apply the nematodes to a wet lawn in the evening (and if your lawn has been recently aerated, even better). The nematodes need to soak into the soil with the water, but they're sensitive to sunlight.